Guard Cell Volume and Pressure Measured Concurrently by Confocal Microscopy and the Cell Pressure Probe,
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چکیده
منابع مشابه
Guard cell volume and pressure measured concurrently by confocal microscopy and the cell pressure probe.
Guard cell turgor pressures in epidermal peels of broad bean (Vicia faba) were measured and controlled with a pressure probe. At the same time, images of the guard cell were acquired using confocal microscopy. To obtain a clear image of guard cell volume, a fluorescent dye that labels the plasma membrane was added to the solution bathing the epidermal peel. At each pressure, 17 to 20 optical se...
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The fluorescent dyes or cellular autofluorescence can be used in studies of cell structure and in physiological measurements. The confocal microscope collects the information from a welldefined optical section; thus, out of focus fluorescence is eliminated, which results in an increase in contrast, clarity and detection of optical objects. Moreover, stacks of optical sections taken at successiv...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 2001
ISSN: 1532-2548,0032-0889
DOI: 10.1104/pp.125.4.1577